Detection of a novel macrophage-derived mucus secretagogue (MMS-68) in bronchoalveolar lavage fluid of patients with asthma
Identifieur interne : 000423 ( France/Analysis ); précédent : 000422; suivant : 000424Detection of a novel macrophage-derived mucus secretagogue (MMS-68) in bronchoalveolar lavage fluid of patients with asthma
Auteurs : Kirk Sperber [France] ; Pascal Chanez [France] ; Jean Bousquet [France] ; Santindra Goswami [France] ; Zvi Marom [France]Source :
- The Journal of Allergy and Clinical Immunology [ 0091-6749 ] ; 1995.
English descriptors
- KwdEn :
- Teeft :
- Airway, Allergic rhinitis, Allergy, Allergy clin immunol april, Allergy clin immunol volume, Alveolar macrophages, Asthma, Asthmatic, Asthmatic group, Asthmatic groups, Asthmatic patients, Asthmatics controls, Balf, Bioassay, Bronchial asthma, Bronchitis, Bronchoalveolar, Bronchoalveolar lavage, Bronchoalveolar lavage fluid, Chronic asthma, Chronic bronchitis, Clin, Constitutive production, Control group, Control groups, Control subjects, Culture supernatants, Cytokine, Elisa, Immunol, Lavage, Macrophage, Measure levels, Mild asthma, Milligram protein, Moderate asthma, Monoclonal antibody, Monocyte, Mucus, Mucus hypersecretion, None none, Normal control subjects, Normal subjects, Novel mucus secretagogue, Other cytokines, Peripheral blood, Protein content, Pulmonary function, Pulmonary macrophages, Respir, Sperber, Standard curves, Statistical difference, Statistical differences, Total cell count, Tumor necrosis factor.
Abstract
Abstract: Background: We have previously described a novel high-molecular-weight macrophage-derived mucus secretagogue (MMS-68) that causes mucuslike glycoconjugate release from cultured airways, nasal explants, and the Ishikawa adenocarcinoma cell line. We have generated monoclonal antibodies against MMS-68 and have developed an antigen-capture ELISA to measure MMS-68 levels in biologic fluids. Using this ELISA, we have demonstrated elevated levels of MMS-68 in the bronchoalveolar lavage fluid (BALF) of smokers and persons chronic bronchitis, in a patient with asthma and bronchorrhea, and in nasal lavage from patients with allergic rhinitis challenged with histamine and methacholine. We have also demonstrated that both spontaneous and lipopolysaccharide induced MMS-68 production is increased in the culture supernatants of monocytes from patients with steroid-dependent asthmas compared with those from normal control subjects. Methods: To delineate further a role for MMS-68 in the regulation of mucus secretion in asthma, we measured MMS-68 levels in the BALF of 37 patients with non-steroid-dependent asthma and of 16 control subjects. Results: There were 21 men and 16 women in the asthma group (age range, 17 to 62 years; mean, 33.8 years) and 11 men and five women in the control group (age range, 18 to 42 years; mean, 27.8 years). There were no statistical differences in either total cell count (145.5 × 103 ± 75.7 cells/mm3 × 103 cells/mm3 vs 134 × 103 ± 65.9 × 103 cells/mm3, p < 0.234) or numbers of alveolar macrophages (103.7 × 103 ± 71.7 × 103/mm3 vs 98.7 × 103 ± 65 × 103 cells/mm 3, p < 0.244) when the asthmatic group was compared with the control group. The MMS-68 level in the asthmatic group was 2.1 ± 0.25 μg MMS-68 per milligram protein compared with 2.09 ± 0.26 μg MMS-68 per milligram protein ( p < 0.256) in the control group. Conclusions: There was no correlation between MMS-68 levels and total protein content, numbers of alveolar macrophages, or the production of other macrophage-derived cytokines including interleukin-1, interleukin-6, or tumor necrosis factor in the asthmatic BALF. Mild asthma, which is clinically not associated with mucus hypersecretion, was not associated with elevated levels of MMS-68. We believe that direct correlation exists between mucus hypersecretion and MMS-68 levels. (J A LLERGY CLIN I MMUNOL 1995;95:868-76)
Url:
DOI: 10.1016/S0091-6749(95)70131-1
Affiliations:
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<front><div type="abstract" xml:lang="en">Abstract: Background: We have previously described a novel high-molecular-weight macrophage-derived mucus secretagogue (MMS-68) that causes mucuslike glycoconjugate release from cultured airways, nasal explants, and the Ishikawa adenocarcinoma cell line. We have generated monoclonal antibodies against MMS-68 and have developed an antigen-capture ELISA to measure MMS-68 levels in biologic fluids. Using this ELISA, we have demonstrated elevated levels of MMS-68 in the bronchoalveolar lavage fluid (BALF) of smokers and persons chronic bronchitis, in a patient with asthma and bronchorrhea, and in nasal lavage from patients with allergic rhinitis challenged with histamine and methacholine. We have also demonstrated that both spontaneous and lipopolysaccharide induced MMS-68 production is increased in the culture supernatants of monocytes from patients with steroid-dependent asthmas compared with those from normal control subjects. Methods: To delineate further a role for MMS-68 in the regulation of mucus secretion in asthma, we measured MMS-68 levels in the BALF of 37 patients with non-steroid-dependent asthma and of 16 control subjects. Results: There were 21 men and 16 women in the asthma group (age range, 17 to 62 years; mean, 33.8 years) and 11 men and five women in the control group (age range, 18 to 42 years; mean, 27.8 years). There were no statistical differences in either total cell count (145.5 × 103 ± 75.7 cells/mm3 × 103 cells/mm3 vs 134 × 103 ± 65.9 × 103 cells/mm3, p < 0.234) or numbers of alveolar macrophages (103.7 × 103 ± 71.7 × 103/mm3 vs 98.7 × 103 ± 65 × 103 cells/mm 3, p < 0.244) when the asthmatic group was compared with the control group. The MMS-68 level in the asthmatic group was 2.1 ± 0.25 μg MMS-68 per milligram protein compared with 2.09 ± 0.26 μg MMS-68 per milligram protein ( p < 0.256) in the control group. Conclusions: There was no correlation between MMS-68 levels and total protein content, numbers of alveolar macrophages, or the production of other macrophage-derived cytokines including interleukin-1, interleukin-6, or tumor necrosis factor in the asthmatic BALF. Mild asthma, which is clinically not associated with mucus hypersecretion, was not associated with elevated levels of MMS-68. We believe that direct correlation exists between mucus hypersecretion and MMS-68 levels. (J A LLERGY CLIN I MMUNOL 1995;95:868-76)</div>
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